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. 2022 Feb 8;9:8. doi: 10.1186/s40580-022-00299-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Morphology of the Caco-2 epithelial cells co-cultured with HUVECs in a static and flow culture condition for 5 days. A Morphological analysis of intestinal specific proteins under a static and flow condition. Scale bars are 50 μm. B Morphological analysis of polarized columnar epithelium. Fluorescence confocal micrographs (vertical cross-sectional views at 5 days after onset) show a vertical cross-section of the epithelium highlighting cell shape and polarity. Horizontal scale bars are 50 μm and vertical scale bars are 25 μm. C The average height of Caco-2 cells grown in the gut-on-a-chip without and with fluidic flow (***p < 0.001)