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. 2022 Jan 26;12:764247. doi: 10.3389/fphar.2021.764247

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Copyright © 2022 Song, Wu, Jiang, Hu, Xu, Tan, Zhang, Yu, Qiu, Wang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of SHI on macrophage polarization in LPS-induced RAW264.7 cells. The cells were treated with SHI (0.5, 1.0, and 2.0 μg/ml). About 2 h later, the cells were stimulated with LPS (5 μg/ml) for 24 h. The relative levels of (A) TNF-α, (B) IL-6, (C) IL-1β, (D) GM-CSF, (E) IL-10, and (F) TGF-β in the supernatant of LPS-induced RAW264.7 cells. (G–I) The cell count of CD16/32 and CD206. (J,K) The ECM1 expression by immunofluorescence. The data are expressed as mean ± SD. Compared with control: ^# p < 0.05, ^## p < 0.01. Compared with LPS: *p < 0.05, **p < 0.01.