FIGURE 5.

The role of ECM1 in SHI-mediated macrophage polarization. The cells were exposed to ECM1 adenovirus added for 24 h. Afterward, the cells were treated with SHI (2.0 μg/ml) for 2 h and then stimulated with LPS for 24 h. (A) The transfection efficiency of ECM1 overexpression. (B–H) The protein expression of ECM1, p-STAT5, STAT5, p-NF-κB, NF-κB, Arg1, CD206, CD16/32, and iNOS. (I–M) The supernatant levels of TNF-α, IL-6, IL-1β, IL-10, and TGF-β in LPS-induced RAW264.7 cells. The data are expressed as mean ± SD. Compared with the other group: *p < 0.05, **p < 0.01.