Bacterial and virus strains |
T4-like Vibrio phage 2.275.O._10N.286.54.E11 |
Martin Polz Lab |
MG592671.1 |
Vibrio cyclitrophicus, strain 10N.286.54.E11 |
Martin Polz Lab |
NZ_MCTE00000000.1 |
Critical commercial assays |
Ribo-Zero Bacteria kit (gram negative and positive) |
Illumina |
discontinued |
Kapa Hyperprep |
Roche |
KK8504 |
Pippin Prep |
Sage Science |
No catalog # |
RNA Clean & Concentrator |
Zymo Research |
R1080 |
TGIRTTM-III enzyme |
InGex |
TGIRT50 |
RNA Ligase 2, truncated KQ |
New England Biolabs |
M0373S |
5´ DNA Adenylation Kit |
New England Biolabs |
E2610L |
Oligo Clean & Concentrator |
Zymo Research |
D4060 |
KAPA SYBR FAST |
Roche |
KK4600 |
MinElute PCR Purification Kit |
Qiagen |
28004 |
Deposited data |
tRNA-seq |
Raw sequencing reads |
PRJNA524872 |
RNA-seq |
Raw sequencing reads |
PRJNA524877 |
Oligonucleotides |
3’ preadenylated adapter AppNNNNAGATCGGAAGAGCACACGTCT/iBiodT/iBiodT/3ddC/ |
This paper |
N/A |
qPCR primers, GroEL, host genome: CAATGGATCTTAAGCGCGGC and CAGAGATAACCGTACCGCCC |
This paper |
Genbank locus tag BCV12_01410 |
qPCR primers, CTP synthetase, host genome: CTTTGGCGATCGTGGTGTTG and TTTTCTAATTCGCCGCGCTG |
This paper |
Genbank locus tag BCV12_03025 |
qPCR primers, GroEL, phage genome: CTTTGAAGACATGGGCGCAC and AACGACTAGGGTTGCAAGCA |
This paper |
Genbank locus tag NVP2275O_355 |
qPCR primers, Major Capsid Protein, phage genome: TGAAGGTGTTATGGGTCGCC and ATACGGGCAGTAGAACGCAG |
This paper |
NVP2275O_445 |
preadenylated DNA adapter AppNNNNGATCGTCGGACTGTAGAACTCTGA/3ddC/ |
This paper |
N/A |
Sequencing primer AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGACGATC |
This paper |
N/A |
Sequencing primer CAAGCAGAAGACGGCATACGAGATBBBBBBGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT (BBBBBB indicates barcode sequence) |
This paper |
N/A |
Software and algorithms |
LocaRNA |
Will S, Reiche K, Hofacker IL, et al. (2007)
|
http://www.bioinf.uni-freiburg.de/Software/LocARNA/
|
tRNAscan-SE |
Lowe TM, Eddy SR (1997)
|
http://lowelab.ucsc.edu/tRNAscan-SE/
|
Aragorn |
Laslett D, Canback B (2004)
|
http://lowelab.ucsc.edu/tRNAscan-SE/
|
MUSCLE |
Edgar RC (2004)
|
https://www.drive5.com/muscle/
|