Table 1.
ISI protocol.
| Experiment stages | Step # | Time (min) | Software used | Description |
|---|---|---|---|---|
| Evoked signal detection (Fig. 1C) | 1 | 5 | Anesthesia, sedation, camera placement | |
| 2 | 10 | primary_script.m secondary_script.m | The camera is focused to the cortex and acquires images of vasculature. A test experiment (green filter and illumination) with a single block of visual stimulus presentation is used to detect global hemodynamic signal | |
| Matrox Intellicam | ||||
| Prep for retinotopic map acquisition | 3 | 0.5 | primary_script.m; secondary_script.m | Adjust focus to intracortical plane (~ 0.1–0.5 mm below cranial surface) |
| 4 | 0.5 | Switch to red filter and illumination | ||
| Azimuth map acquisition | 5 | 30.5–61 | primary_script.m; secondary_script.m | Load horizontal.param in primary_script.m and run script for visual stimulation in azimuth |
| Elevation map acquisition | 6 | 30.5–61 | Load vertical.param in primary_script.m and run script for visual stimulation in elevation | |
| Retinotopic map analysis and display (Fig. 1F; Sup. Fig. S3) | 7 | 10–20 | run_first.m | Load Post-imaging folder and run run_first.m to construct retinotopic and VFS maps. Processing includes: |
| – Retrieval of slow fluctuations in intensity across all imaging frames (frequency domain) | ||||
| – Azimuth and elevation phase maps for each trial | ||||
| – SNR thresholding | ||||
| – Average azimuth and elevation retinotopic maps per session | ||||
| – Combination of maps from multiple imaging sessions | ||||
| – Overlay of retinotopic, VFS, vasculature | ||||
| Alignment of craniotomies to retinotopic contours (Fig. 4B) | 8 | 5 | Align.m | Load Post-imaging folder and run Align.m to align craniotomy images to retinotopic contours |