Table 1:

Studies included in meta-analysis for estimating the sensitivities and specificities of LAMP and PCR methods

Reference	Country	Microorganism	All samples	Food samples	Detection methods	Sensitivity	Spec ificity	Results
								T	F	F	T
								P	N	P	N
17	United Kingdom	Campylobacter jejuni	97 samples	Raw poultry meat, offal, raw shellfish, and milk samples	qPCR	100	85	57	0	6	34
				Raw poultry meat, offal, raw shellfish, and milk samples
18	China	Salmonella strains Non-Salmonella strains	85 samples	Minced meat of pig raw milk	LAMP	100	100	15	0	0	70
19	China	Escherichia coli	36 samples	Eggs, raw sausage, salmon, ham, cooked ham, bacon, chicken, beef, pork, duck, hard cheese, raw-milk	Multiplex PCR	100	80	52	1	2	53
		Listeria monocytogenes				100	100
		Salmonella spp.				92.30	95.65
20	Iran	Escherichia coli	18 samples	Eggs, raw milk, Raw Kobide, salad, chicken, cheese	Multiplex PCR	100	100	40	0	1	13
		Listeria monocytogenes				100	100
		Salmonella spp.				100	80
21	Egypt	Listeria monocytogenes	66	Clinical samples	PCR	100	98.72	9	0	2	15
			100	food samples							5
22	China	Listeria monocytogenes	2 reference strains	Chicken samples	PCR	71.42	100	5	2	0	53
			10 target strain
			10 non-listeria strains
			60 chicken samples		LAMP	100	100	7	0	0	53
23	Louisiana, USA	Shiga toxin-producing Escherichia coli (STEC)	50 STEC strains	Ground beef	Stx1-LAMP	100	100	11	0	0	37
			40 non-STEC strains
					Stx2-LAMP	100	100	3
					Stx2-LAMP	100	100
24	Japan	Verotoxin-producing bacteria, Salmonella, Shigella	50 Mixed human feces	NA*	PCR	100	100	1	0	0	49
25	China	Listeria monocytogenes	182 Strains	Various food samples	LAMP	96.70	100	176	6	0	39
					PCR	91.20	100	166	16	0	39
26	USA	Staphylococcus spp.	118 clinical isolates	NA	LAMP	98	100	249	5	0	101
					PCR	92.49	100	234	19	0	101
27	Italy	Salmonella	175 samples (102 spiked samples and 73 real samples)	Minced meat and meat preparations made from poultry meat intended to be eaten cooked	qPCR	100	100	100	0	0	75
28	Canada	Shiga toxin-producing Escherichia coli (STEC)	632 stool samples from pediatric patients	NA	qPCR	100	100	21	0	0	611
29	Japan	E. Coli serovars, Listeria monocytogenes serovars, Shigella spp. Salmonella spp. Vibrio cholerae, Campylobacter spp. Clostridium perfringens, Legionella spp.	6 Human fecal samples 40 Environmental water samples	NA	qPCR	100		6	0	0	0
30	China	48 V. Parahaemolyticus and 10 non- V. Parahaemolyticus strains	Seafood Samples	20 fish, 10 shrimp, and 18 mussel samples and 10 non-V. Parahaemolyticus strains	PCR	89.58	100	43	5	0	10
					LAMP	96.87	100	93	3	0	20
31	China	Salmonella enterica	Artificial Contamination of Raw Milk	Raw milk	LAMP	94.79	100	91	5	0	154
32	China	Salmonella enterica subsp. Enterica Listeria monocytogenes Escherichia coli O157 Vibrio parahaemolyticus V. Vulnificus Campylobacter jejuni Enterobacter sakazakii Shigella spp.	Spiked stool samples	NA	Multiplex qPCR	100	99.87	1	0	34	28
								9			01
								9			6
								5
33	USA	C. Jejuni, V.fluvialis, V. Mimicus, V. Metschnikovii, V. Cholerae, ETEC, V. Parahaemolyticus, C. Coli V. Furnissii, EIEC, EPEC Y. Enterocolitica, DAEC Shigella spp Salmonella spp. S. Typhi L. Monocytogenes C. Lari STEC	97 stool and other clinical samples	NA	PCR	98.11	99.75	9	1	4	17
								3	8	4	79
								8			8
34	China	61 V. Parahaemolyticus strains, 34 non-target strains	70 seafood samples	All Samples	LAMP	100	100	11	0	0	59
				Sleevefish, Oyster, Jellyfish, Weever, Shrimp, Tegillarca, Cuttlefish (n=10)	PCR	90.90	100	10	1	0	129
35	China	VBNC, Enterohemorrhagic E. Coli	Enterohemorrhagic E. Coli strain, ATCC43895 and 6 E. Coli strains	Various food samples during 2003–2007	LAMP	100	100	0	0	7	0
36	Canada	31 strains of both Gram-negative and Gram-positive bacteria (Pseudomonas aeruginosa ATCC 9721, Listeria monocytogenes, Staphylococcus aureus, Campylobacter jejuni ATCC 33560, Campylobacter coli)	Standard Strains	Samples of fresh produce	LAMP	100	100	16	0	0	8
37	China	S. Aureus, Salmonella, and Shigella, L. Monocytogenes	17standard strains were used for specificity and sensitivity testing	Artificially contaminated juice	Multiplex LAMP	Sensitivity of mlam p was 10- fold high er than mpcr	100	17	0	0	0
38	Thailand	Staphylococcus aureus	40 milk samples and 40 Pork samples	40 ground pork and 40 milk samples	LAMP	100	100	7	0	0	33
						100	97	5	0	1	34
39	Germany	Salmonella spp.	180 bacterial Strains, 88 tested Salmonella strains, 92 tested non-Salmonella strains	RTE salad and Chicken carcass Minced meat Artificial contamination of food samples	LAMP	100	100	88	0	0	92

^*:Not Applicable, no food samples were evaluated in the study