Fig. 5. Relationships between changes in dissolved gases and medium acidification in cultures of H1 hESC, K562, and GM12878.

Delta (∆) values for the environmental variables (pH, dO₂, and dCO₂) represent the mean difference between levels measured at 8 h intervals minus measurements taken at time zero. a The relationship between reductions in pH and increases in dCO₂ in cell culture media. b The relationship between reductions in dO₂ and increases in dCO₂ in cell culture media. c The relationship between reductions in pH and dO₂ in cell culture media. Data points represent individual measurements taken from three culture flasks per cell line. Insets show the Pearson correlation coefficient (r) and p-value for each correlation model (cf. Supplementary Table S2 for full statistical results).