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. 2022 Feb 8;8:56. doi: 10.1038/s41420-022-00837-5

Fig. 2. mTOR activity during early stages of RO development.

Fig. 2

Quantitative RT-PCR of A total mTOR, B Raptor, C Rictor, and D S6K1 using ROs at indicated days of development. Immunofluorescence staining of HuC/D (green) and p-S2448 (red) (E) or p-S6 (red) (F) of RO at indicated time points. After 35 days of differentiation, both p-S2448 and p-S6 expressions in ROs showed upregulation mainly in the inner layers which co-localized with HuC/D expression, while more robust expressions were found in the outer layers as well as the inner layers of ROs after 40 days of differentiation. Limited p-S2448 and p-S6 activities in outer layers of ROs with scarce co-localization with HuC/D+ cells were noted after 50 days of differentiation. **p < 0.01, by the Kruskal–Wallis test with post hoc analysis. Scale bar, 50 μm.