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. 2022 Feb 8;12:2120. doi: 10.1038/s41598-022-04803-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The intensity of Raman peak centered at 1584 cm⁻¹ as a function of Cyt c concentration (raw data) for: (A) the oxidized form of Cyt c, experimental conditions the same as for breast single cells measurements: integration time 0.5 s, 1 accumulation, laser power 10 mW; (B) the oxidized form of Cyt c, experimental conditions the same as for breast tissue measurements: integration time 1.0 s, 1 accumulation, laser power 10 mW; (C) the reduced form of Cyt c, experimental conditions the same as for breast single cells measurements: integration time 0.5 s, 1 accumulation, laser power 10 mW, reduction agent NaBH₄ in tenfold excess; (D) the reduced form of Cyt c, experimental conditions the same as for breast tissue measurements: integration time 1.0 s, 1 accumulation, laser power 10 mW, reduction agent NaBH₄ in tenfold excess.