Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 8;12:2133. doi: 10.1038/s41598-022-05882-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Comparison of ddPCR to real-time PCR for quantitation of cccDNA. (A) The plasmid containing the HBV genotype C2/Ce DNA genome (AB246345) was measured using a tenfold dilution of DNA ranging from 10⁵ to 10⁰ copies/assay by ddPCR or real-time PCR. (B) Intrahepatic cccDNA levels in the liver of chimeric mouse infected with HBV were measured using various amounts of DNA ranging from 20 to 0.156 ng/assay by ddPCR or real-time PCR. (C) The intrahepatic cccDNA levels measured by ddPCR and real-time PCR revealed substantial agreement. (D) Intrahepatic cccDNA levels from the liver of chimeric mouse with HBV infection were measured using various amounts of DNA, ranging from 8 to 5 ng/assay. (E) Intrahepatic HBV DNA and cccDNA levels in the liver of chimeric mouse infected with HBV were measured using various amounts of DNA ranging from 20 to 0.02 ng/assay by ddPCR or real-time PCR. Each assay was conducted in triplicate. Target concentrations were repeatedly and independently tested three times. (F) The comparison of the HBV plasmid using a tenfold dilution of DNA ranging from 10⁵ to 10⁰ copies/assay with or without the sera from IC patient (#9 in Fig. S3C) by ddPCR. The cccDNA signal in the absence of the HBV plasmid was negative irrespective of the sera.