FIGURE 7.

The protein levels of PTPN7, total ERK1/2, and phosphorylated ERK1/2 in boar sperm were measured by Western blot. (A) Anti-phosphorylated proteins were extracted from transfected, frozen–thawed, and capacitated sperm. Western blots performed using anti-ERK1/2, phospho-ERK1/2, PTPN7, and β-Tubulin antibodies. The results are representative of three independent experiments. (B) The protein levels of phosphorylated ERK1/2 and PTPN7 in fresh, frozen–thawed, NC, and capacitated sperm. “NC” means negative control group incubated at 37°C. “Capa-” means capacitation group. The protein level of phosphorylated ERK1/2 was quantified by normalization to total ERK1/2 protein using ImageJ, and the level of PTPN7 was quantified by normalization to β-Tubulin. (C) The protein levels of phosphorylated ERK2 and PTPN7 were shown in transfected sperm at 6 h.