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. 2022 Jan 26;12:785699. doi: 10.3389/fpls.2021.785699

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Copyright © 2022 De Kesel, Degroote, Nkurunziza, Singh, Demeestere, De Kock, Anggraini, Matthys, Wambacq, Haesaert, Debode and Kyndt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cucurbitaceae COld Peeling Extracts (CCOPEs) are promising candidates to trigger induced resistance (IR). Rice cell suspension cultures (RCSCs) were incubated for 4 h with the buffer used for CCOPE preparation (Control) or CCOPE derived from pumpkin (Cucurbita moschata cv. Musquee de Provence; pCOPE), zucchini (C. pepo var. cylindrica; zCOPE) or melon (Cucumis melo var. cantalupensis; mCOPE). Expression levels of the six screening genes identified by De Kesel et al. (2020) were determined by RT-qPCR and are expressed relative to the mock-treated cells. Error bars indicate the 95% confidence interval. Asterisks indicate statistically significant differences upon comparison of CCOPE- and mock-treated cells. Statistical results and 95% confidence intervals were obtained via the Rest2009 software (Pfaffl, 2001) (p < 0.05).