TABLE 1.
Foliar treatment with Cucurbitaceae COld Peeling Extract (CCOPE) derived from melon (Cucumis melo var. cantalupensis; mCOPE) leads to systemic defense induction in rice.
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Terms upregulated at 1 dpt
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MapMan
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| Bin ID | Bin name | p-value |
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| 10 | Cell wall | <1 × 10–20 |
| 26.12 | Peroxidases | 1.43 × 10–5 |
| 20.1 | Biotic stress | 3.13 × 10–4 |
| 27.3.32 | WRKY transcription factors | 5.07 × 10–3 |
| 17.3.32 | Hormone metabolism ethylene ACC synthase | 2.33 × 10–2 |
| 17.7 | Hormone metabolism jasmonate | 3.10 × 10–2 |
| 21.2 | Glutathione-ascorbate redox system | 4.24 × 10–2 |
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| Gene ontology | ||
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| Term ID | Term name | p-value |
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| GO:0071554 | Cell wall organization or biogenesis | 1.05 × 10–6 |
| GO:0009698 | Phenylpropanoid metabolic process | 1.30 × 10–2 |
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| KEGG pathway | ||
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| Term ID | Term name | p-value |
| KEGG:04626 | Plant–pathogen interaction | 1.84 × 10–4 |
| KEGG:00940 | Phenylpropanoid biosynthesis | 3.52 × 10–4 |
| KEGG:01110 | Biosynthesis of secondary metabolites | 2.80 × 10–2 |
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| Terms upregulated at 4 dpt | ||
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| MapMan | ||
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| Bin ID | Bin name | p-value |
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| 26.12 | Peroxidases | 2.42 × 10–9 |
| 27.3.32 | WRKY transcription factors | 7.38 × 10–5 |
| 16.2 | Secondary metabolism phenylpropanoids | 8.28 × 10–5 |
| 10.7 | Cell wall modification | 2.84 × 10–3 |
| 17.5.1.1 | Hormone metabolism ethylene ACC synthase | 5.89 × 10–3 |
| 20.1 | Biotic stress | 8.17 × 10–3 |
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| Gene ontology | ||
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| Term ID | Term name | p-value |
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| GO:0042744 | Hydrogen peroxide catabolic process | 2.93 × 10–12 |
| GO:0071669 | Plant-type cell wall organization or biogenesis | 1.74 × 10–4 |
| GO:0006950 | Response to stress | 2.94 × 10–4 |
| GO:0031347 | Regulation of defense response | 4.27 × 10–2 |
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| KEGG pathway | ||
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| Term ID | Term name | p-value |
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| KEGG:00940 | Phenylpropanoid biosynthesis | 1.51 × 10–9 |
| KEGG:04626 | Plant–pathogen interaction | 9.73 × 10–7 |
| KEGG:01110 | Biosynthesis of secondary metabolites | 3.18 × 10–2 |
The rice root transcriptome was investigated 1 and 4 days post mCOPE treatment (1 and 4 dpt, respectively) and compared with buffer-treated control plants. Upon treatment, all plants were 14 days old. This table shows a selection of defense-associated terms obtained via MapMan (Thimm et al., 2004) and gene ontology (GO)-enrichment (Reimand et al., 2016) analyses on significantly upregulated genes after comparing gene transcription levels of mCOPE- vs. mock-treated plants. ACC, 1-aminocyclopropane-1-carboxylic acid, an ethylene precursor. Per-gene transcription levels and complete outcomes of MapMan and GO-enrichment analyses can be found in Supplementary Tables 1, 2, respectively.