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. Author manuscript; available in PMC: 2022 Feb 9.
Published in final edited form as: J Phys Chem B. 2021 Dec 28;126(1):110–122. doi: 10.1021/acs.jpcb.1c09048

Table 1.

Selected Photophysical Properties of Each Component Fluorophore

QY ε (M−1 cm−1) λ Absmax (nm) λ Emmax (nm)
AF405 72 ± 5.1%a 35 000 401 421
PIC (monomer/J-bit) 0.012/0.18%b 53 500 525 584
AF647 41 ± 2.6%c 270 000 650 665
calculated spectral overlap integral (J, nm4 M−1 cm−1)/R0 (nm)
PIC (monomer) AF647
AF405 4.3 × 1014/3.8 7.8 × 1012/1.9
PIC (monomer) 6.9 × 1013/0.7 1.3 × 1016/2.4
a

AF405 QY measured relative to quinine sulfate in 0.1 N H2SO4.30

b

PIC monomer and J-bit QY values are from literature values, ε, λ Absmax, and λEmmax refer to the PIC monomer as reported.20

c

AF647 QY was measured relative to Oxazine 710 in methanol.31 Spectral overlaps were calculated using measurements of each individual dye component, examples in Figure 1A.