Skip to main content
. Author manuscript; available in PMC: 2022 Feb 9.
Published in final edited form as: J Phys Chem B. 2021 Dec 28;126(1):110–122. doi: 10.1021/acs.jpcb.1c09048

Table 2.

Predicted Dye Separation Distances (nm)/Estimated FRET Efficiencya

name D405 → A647 D405 → PIC PIC → A647b extension of AT-trackc
AT10 9.5/0% 3.0/82% 3.0/26.8% 3.4
AT20 12.9/0% 3.0/82% 3.0/26.8% 6.8
AT30 16.3/0% 3.0/82% 3.0/26.8% 10.2
OV 13.6/0% 3.0/82% 2.4/62.4% 8.1
CO 16.3/0% 3.0/82% 3.0/26.8% 10.2
GC 9.5/0% N/A N/A N/A
a

N/A = Not applicable (GC-tracks do not specifically bind PIC leading to uncertainty in the placement of the initial or final PIC monomers). → = FRET step.

b

Estimated FRET efficiency based on using PIC QY measured in DNA from ref 18.

c

PIC homo-FRET through AT-track discussed in the Results and Discussion section.