| Antibodies |
|
|
|
| Rabbit anti- YAP1 (D8H1X) |
Cell Signaling |
Cat# 14074 |
| Rabbit anti- cleaved caspase 3 |
Cell Signaling |
Cat# 9661 |
| Rabbit anti- p-histone H3 |
Cell Signaling |
Cat# 3377 |
| Rabbit anti- Olfm4 |
Cell Signaling |
Cat# 39141 |
| Mouse anti- E-cadherin |
BD Biosciences |
Cat# 610182 |
| Mouse anti- beta-catenin |
BD Biosciences |
Cat# 610153 |
| Mouse anti- alpha E-catenin |
Santa Cruz |
Cat# SC-9988 |
| Rabbit anti- Pcna |
Santa Cruz |
Cat# SC-56 |
| Rabbit anti- Na+K+ATPase |
MBL |
Cat# MC-9784 |
| Rabbit anti- Ki67 |
Abcam |
Cat# AB16667 |
| Rabbit anti- Lysozyme |
Dako |
Cat# A0099 |
| Rabbit anti- Cdc42 |
Cell Signaling |
Cat# 2466 |
| Rabbit anti- phospho-YAP (S127) |
Cell Signaling |
Cat# 13008 |
| Rabbit anti-phospho-MST1 (Thr183)/MST2 (Thr180) |
Cell Signaling |
Cat# 49332 |
| Rabbit anti- MST 1/2 |
Bethyl Laboratories |
Cat# A300-468A |
| Rabbit anti- phospho-S6 Ribosomal Protein (S235/236) |
Cell Signaling |
Cat# 4858 |
| Mouse anti- S6 Ribosomal Protein |
Cell Signaling |
Cat# 2317 |
| Rabbit anti- phospho-4E-BP1 (T37/46) |
Cell Signaling |
Cat# 2855 |
| Rabbit anti- 4E-BP1 |
Cell Signaling |
Cat# 9644 |
| Rabbit anti- K48-linkage polyubiquitin |
Cell Signaling |
Cat# 8081 |
| Rabbit anti- phosphor-EGFR (Y1068) |
Cell Signaling |
Cat# 3777 |
| Rabbit anti- EGFR |
Cell Signaling |
Cat# 2232 |
| Rabbit anti- beta-actin |
Cell Signaling |
Cat# 4970 |
| Rabbit anti- phosphor-Lats-1/2 (Ser909, Ser872) |
Invitrogen |
Cat# PA5-64591 |
| Rabbit anti- Lats 1/2 |
Bethyl Laboratories |
Cat# A300-479A |
| Mouse anti- Scribble |
Santa Cruz |
Cat# SC-55543 |
| Anti-mouse IgG, HRP-linked Antibody |
Cell Signaling |
Cat# 7076 |
| Anti-rabbit IgG, HRP-linked Antibody |
Cell Signaling |
Cat# 7074 |
| Alexa Fluor 488, Goat anti Rabbit IgG |
Invitrogen |
Cat# A32731 |
| Alexa Fluor 568, Goat anti Rabbit IgG |
Invitrogen |
Cat# A11011 |
| Alexa Fluor 633, Goat anti Rabbit IgG |
Invitrogen |
Cat# A21070 |
| Alexa Fluor 488, Goat anti Mouse IgG |
Invitrogen |
Cat # A32723 |
| Alexa Fluor 568, Goat anti Mouse IgG |
Invitrogen |
Cat# A11004 |
| Alexa Fluor 633, Goat anti Mouse IgG |
Invitrogen |
Cat# A21052 |
| Normal Rabbit IgG |
Cell Signaling |
Cat #2729 |
| Normal Mouse IgG |
Sigma |
Cat #12-371 |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Tamoxifen |
Cayman Chemical |
13258 |
| Rapamycin |
ApexBio |
A8167 |
| Afatinib |
ChemieTek |
CT-BW2992 |
| Pierce Phosphatase Inhibitor Mini Tablets |
Thermo Fisher |
A32957 |
| Simple Stop 1 Phosphatase Inhibitor Cocktail |
Gold Biotechnology |
GB450 |
| complete, Mini, EDTA-free Protease Inhibitor Cocktail |
Roche |
11836170001 |
| Alcian Blue |
Sigma |
A3157 |
| DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) |
Invitrogen |
D1306 |
|
| Critical commercial assays |
|
|
|
| High-Capacity cDNA Reverse Transcription Kit |
Applied Biosystems |
4368814 |
| TaqMan Gene Expression Master Mix |
Applied Biosystems |
4369514 |
| TaqMan Gene Expression Master Mix |
Applied Biosystems |
4369514 |
|
| Deposited data |
|
|
|
| Single Cell RNA-seq data |
This paper |
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE166736
|
|
| Experimental models: Organisms/strains |
|
|
|
| Mouse: Olfm4-IRES-eGFPCreERT2 |
Schuijers et al., 2014
|
N/A |
| Mouse: CDC42flox/flox |
Melendez et al., 2013
|
N/A |
| Mouse: villin-creERT2 |
Melendez et al., 2013
|
N/A |
| Mouse: Rosa26tdTomato |
Madisen et al., 2010
|
JAX # 007909 |
| Mouse: Yapflox/flox; Tazflox/flox |
Xin et al., 2013
|
N/A |
| Mouse: Scribbleflox/flox |
Hartleben et al., 2012
|
N/A |
|
| Oligonucleotides |
|
|
|
| Primers for qPCR (Table S1) |
N/A |
N/A |
|
| Software and algorithms |
|
|
|
| ImageJ |
National institutes of Health and the Laboratory for Optical and Computational Instrumentation |
https://imagej.nih.gov/ij/
|
| Prism 8.1 |
GraphPad Software |
https://www.graphpad.com/scientific-software/prism/
|
| Cell Ranger 1.3.0 |
10x Genomics |
https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/1.3
|
| R |
R Foundation for Statistical Computing |
https://www.r-project.org/
|
