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. 2022 Jan 3;39(2):msab370. doi: 10.1093/molbev/msab370

Fig. 6.

Fig. 6.

Cellulases of Pristionchus pacificus increase nematode fitness on bacterial cellulosic biofilms. (A) An illustration of assays for testing brood size in P. pacificus. (B) Brood size of P. pacificus wild type (wt) and octuple mutant (oct) using Escherichia coli OP50 as the food source under nutrient-limited conditions supplemented with 0.5% CMC (one-tailed Mann–Whitney rank test). (C) Brood size of P. pacificus wild type (wt) and octuple mutant (oct) on different E. coli K-12 strains under the standard NGM condition (one-tailed t-test). (D) Brood size of P. pacificus wild type (wt) and octuple mutant (oct) under nutrient-reduced condition, showing an enhanced reproductive potential of wild-type animals on E. coli K-12 cellulose + (one-tailed t-test). (E) (Left) Assays for measuring developmental speed. (Right) Under the standard NGM condition, P. pacificus wild type (wt) and octuple mutant (oct) have a similar developmental speed on E. coli OP50, whereas the wild type develops faster than the octuple mutant on E. coli K-12 strains. The developmental speed of offspring from multiple hermaphrodites was determined (PERMANOVA; examined plates between 14 and 28). * indicates P < 0.05. All experiments were performed in duplicate. Nutrient conditions are summarized in supplementary table S3, Supplementary Material online.