TABLE 2.

Oncolytic viruses that dismantle the tumor microenvironment to improve virus dissemination.

Virus backbone	New virus	Modification	Reason	Result	Reference
Adenovirus	Ad-ΔE1B-RLX	Addition of relaxin to Ad-ΔE1B	Improve virus distribution in the tumor	Reduced lung metastasis in a mouse melanoma model	Kim et al. (2006)
Adenovirus	OV-RLX-5T35H	Addition of relaxin to Ad5/35 adenovirus	Improve virus distribution in the tumor	Reduced metastasis and improved survival in a mouse pancreatic tumor model	Ganesh et al. (2007)
VV a	GLV-1h255	Addition of MMP-9 to GLV-1H68	Improve virus distribution in the tumor	Did not alter metastasis in a mouse prostate cancer model	Schäfer et al. (2012)
Adenovirus	VCN-01	Addition of hyaluronidase (PH20) into fiber-modified AdΔ24E1A	Improve virus distribution in the tumor	Reduced metastasis in a metastatic osteosarcoma mouse model	Martínez-Vélez et al. (2016)
Adenovirus	EnAdDNAse	Addition of exonuclease DNAse I into ColoAd1 Enadenotuvirev	Improve virus distribution in the tumor	Reduced tumor growth and improved virus spread	Tedcastle et al. (2016)
Adenovirus	EnAdPH20	Addition of hyaluronidase into ColoAd1 Enadenotuvirev	Improve virus distribution in the tumor	Reduced tumor growth and improved virus spread	Tedcastle et al. (2016)
Reovirus	S1-T241I	Mutation of viral binding protein sigma1 to impede cleavage by tumor proteases	Improve virus distribution in the tumor	Improved virus distribution in primary tumors	Fernandes et al. (2019)

^aVV, vaccinia virus.