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. 2021 Dec 10;39(2):msab353. doi: 10.1093/molbev/msab353

Fig. 8.

Fig. 8.

Histological analysis, mRNA, and miRNA expressions and genetic mechanisms for the gene IRF2BP2 and oar-miR-20a-3p involved in the fleece fiber variation in domestic sheep. (A) Expressions of IRF2BP2 in embryonic developmental stages and 2 years old of fine-wool (left), coarse-wool sheep (medium) and boxplot of FPKM distribution between fine-wool and coarse-wool sheep (right). (B) Expressions of miRNAs in coarse-wool sheep (Hu sheep) normalized by reads per million reads mapped to miRNAs (RPM). (C) Putative binding sites for oar-miR-20a-3p to the 3′-UTR of IRF2BP2. (D) Dual-luciferase reporter assays, *P < 0.05 (t-test); N.S., not significant. (E) Proposed genetic mechanisms for IRF2BP2 and oar-miR-20a-3p involved in the fleece fiber variation in domestic sheep. ①Ho et al. (2012) and ②Zhang et al. (2018). (F) Histological transverse section of skin in Chinese Merino (fine wool, at embryonic 85 days) and Wadi sheep (coarse wool, at embryonic 90 days); PF, primary wool follicle; SF, secondary wool follicle.