Skip to main content
. 2022 Jan 26;9:806966. doi: 10.3389/fmolb.2022.806966

FIGURE 4.

FIGURE 4

In vitro interference of miR-135b reduces ranolazine inhibition of cardiac fibroblast pyroptosis. (A) Binding targets between miR-135b and caspase-1. (B) Real-time PCR detection of changes relative to miR-135b expression in groups. (C,D) Real-time PCR was performed to detect the relative mRNA expression of caspase-1 and IL-1β in cardiac fibroblasts in the groups. (E–G) Western blot detection of the relative protein expression of caspase-1, IL-1β and GSDMD in cardiac fibroblasts. (H–J) Immunofluorescence detection of caspase-1, IL-1β and GSDMD expression in cardiac fibroblasts. Scale bar = 50 μm *** p < 0.001 vs. NG + AMO-NC,*** p < 0.001 vs. NC, ## p < 0.01 vs. HG + AMO-NC, ### p < 0.001 vs. HG + AMO-NC, $ p < 0.05 vs. HG + Ran + AMO-NC, $$ p < 0.01 vs. HG + Ran + AMO-NC, $$$ p < 0.001 vs. HG + Ran + AMO-NC; n = 3.