Figure 2.

Human one-cell upregulated gene characteristics and pathways

(A) Raw scRNA-seq density plots (Sashimi plots) along exons and exon junctions. Arcs representing splice junctions connecting exons and display the number of reads split across the junction (junction depth) in mII oocytes (mII) and 2PN one-cell embryos (emb). Genomic coordinates (chrom) and gene annotation tracks are aligned beneath each respective plot. Solid black bars above plots indicate regions of potential alternative splicing.

(B) qPCR for transcripts in individual human monopronuclear (1PN) and tripronuclear (3PN) one-cell embryos (3 ≥ n ≥ 6 biologically independent oocytes or embryos per target). Values for metaphase II oocytes (mII) and bipronuclear one-cell embryos (2PN) from Figure 1E are included for comparison. Values are ± SEM and normalized against mII oocytes (∼1.0). Unpaired t tests indicate p < 0.2.

(C) Ingenuity pathway analysis (IPA) of gene networks upregulated (FDR < 0.1, log₂FC > 0) in 2PN one-cell embryos.

(D–F) Upstream transcription regulators inferred by IPA of upregulated gene networks (FDR < 0.1, log₂FC > 0) in 2PN one-cell embryos for E2F4 (D), MYC (E), and MYCN (F).

See also Figure S2 and Table S1.