REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Human metaphase II (mII) oocytes	Ovation Fertility Austin, Embryology and Andrology Laboratories, Austin, TX 78731, USA	https://www.ovationfertility.com
Human bipronuclear (2PN) embryos	Ovation Fertility Austin, Embryology and Andrology Laboratories, Austin, TX 78731, USA	https://www.ovationfertility.com
Human monopronuclear (1PN) embryos	Ovation Fertility Austin, Embryology and Andrology Laboratories, Austin, TX 78731, USA	https://www.ovationfertility.com
Human tripronuclear (3PN) embryos	Ovation Fertility Austin, Embryology and Andrology Laboratories, Austin, TX 78731, USA	https://www.ovationfertility.com
Critical commercial assays
Clontech SMARTer Total RNA-Seq Kit Pico Input (V2) system	Takara Clontech	Cat # 634412
Deposited data
RNA-seq data	This paper	GEO: GSE157834
RNA-seq data	Leng et al., 2019	GEO: GSE133856
RNA-seq data	Xue et al., 2013	GEO: GSE44183
RNA-seq data	Wu et al., 2018	GEO: GSE101571
RNA-seq data	Sun et al., 2021	GEO: GSE137490
Experimental models: Organisms/strains
Human (Homo sapiens)		N/A
Oligonucleotides
Primers for qPCR: see Table S2	This paper	N/A
Software and algorithms
Human GRCh38 genome and Ensembl 92 gene model using STAR (2.5.0a)	Dobin et al., 2013	N/A
Stringtie (90 1.3.6)	Pertea et al., 2015	N/A
htseq-count (0.6.1p1)	Anders et al., 2015	N/A
edgeR	Robinson et al., 2010	N/A
trim-mean of M values (TMM) normalization from the edgeR package	Robinson and Oshlack, 2010	N/A
Rtsne package		https://cran.r-project.org/web/packages/Rtsne/Rtsne.pdf
limma package	Ritchie et al., 2015	N/A
Qiagen Ingenuity Pathway Analysis (IPA) software		https://digitalinsights.qiagen.com/products/qiagen-ipa/latest-improvements/current-line/
Sashimi plots	Katz et al., 2010; Wang et al., 2008	N/A
Integrative Genome Viewer (IGV) version 2.4.19	Robinson et al., 2011	N/A