FIG 6.

Hsp90 inhibition affects protein-RNA interactions in Leishmania. (A) Schematic representation of combination of Hsp90 inhibition with tanespimycin treatment and the OOPS-quantitative proteomic MS method for proteome-wide quantitative assessment of the effect of the inhibition on protein-RNA interactions in both promastigote and axenic amastigote life cycle stages of L. mexicana. For the Hsp90 inhibition, the parasites in three independent replicates were treated with 1 µM tanespimycin or vehicle (DMSO) for 16 h prior to the OOPS protocol. Volcano plots showing differential regulation of RBPs upon tanespimycin treatment (+Tan) with respect to vehicle treatment (-Tan) in promastigotes (B) and axenic amastigotes (C). A modified t test with permutation-based FDR statistics (250 permutations, FDR = 0.05) was applied to compare the +Tan and -Tan groups in both life cycle stages. (D) Top-10 Pfam domain occurrences in tanespimycin-affected RBPs in promastigotes and axenic amastigotes.