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. Author manuscript; available in PMC: 2022 Feb 9.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2016 Dec 9;1862(3):324–336. doi: 10.1016/j.bbalip.2016.12.004

Fig. 2.

Fig. 2.

RhoprGpat1 and RhoprGpat4 are differentially expressed. At the fourth day after blood meal, anterior midgut (AM), posterior midgut (PM), fat body (FB) and ovary (OV) were dissected from adult females and RhoprGpat1 and RhoprGpat4 mRNA expression levels were determined by qPCR (A). Alternatively, anterior midgut (B), posterior midgut (C) and fat body (D) were obtained from adult females before feeding (day 0) or on different days after blood meal. Follicles of different lengths (E) were isolated from females on day 4 after a blood meal. Differences between RhoprGpat4 cDNA levels were determined by qPCR analysis. mRNA expression levels are relative to RhoprGpat4 transcripts in the anterior midgut (A), in unfed insects (B–D) or 0.5 mm follicles (E), set as 1.0. The results represent the means ± S.E.M. of 3–4 independent experiments. Where not seen, error bars are smaller than the symbols. (***): P < 0.001 compared to RhoprGpat1 values by one-way ANOVA.