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. Author manuscript; available in PMC: 2022 Feb 9.
Published in final edited form as: Sci Signal. 2020 Sep 29;13(651):eaba3300. doi: 10.1126/scisignal.aba3300

Fig. 4. P2Y11 receptors restrict excitatory P2X4 receptor signaling to the front of migrating T cells.

Fig. 4.

Jurkat cells expressing EGFP-tagged P2X4 receptors were labeled with MitoTracker Red CM-H2Xros and treated with P2Y11 antagonist (NF340, 10 μM), P2Y11 agonist (NF546, 1 μM), or vehicle control for 10 min. Then, cells were stimulated with SDF-1α (100 ng/ml) and redistribution of P2X4 receptors and active mitochondria was analyzed after 3 min. Representative images are shown in panel A. Summarized results of 6-10 separate experiments are shown in panel B. Dots indicate the distribution of single cell fluorescence values and lines represent averaged summarized line profiles of the whole cell areas. Scale bar: 10 μm.