FIG 6.

Activation of ATF6-mediated unfolded protein response and AAV2 transduction in monkey primary hepatocytes. (A) Reporter assays for ATF6, CHOP, and XBP1 activation in monkey primary hepatocytes following AAV2 infection for 6 h. (B) Western blot and densitometric quantification of ATF6, BIP, and GRP94 during AAV2 infection time courses. (C) Luciferase reporter assays showing ATF6, CHOP, and XBP1 activation. Monkey primary hepatocytes were transfected with AAVR-targeted short hairpin RNA (shRNA) or a scrambled control(sh-scream) for 24 h. (D) Reporter assays showing ATF6 activation in monkey primary hepatocytes treated with reagents. (E) AAV2 infection of a luciferase reporter in monkey primary hepatocytes. Data are presented as the mean ± standard deviation (n ≥ 3). Statistical significance was determined using the unpaired Student’s t test. *, P < 0.05; **, P < 0.01.