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Chromatin immunoprecipitation (ChIP) and PCR amplification of HVEM promoter. 293T cells were transfected with WT sncRNA1-FLAG or WT sncRNA2-FLAG as described in Materials and Methods. Chromatin from transfected cells was sheared and immunoprecipitated with anti-FLAG beads. Immunoprecipitated chromatin and input chromatin were amplified with primers corresponding to HVEM promoter sequences. Experiments were repeated twice.
