FIG 2.

sncRNA1 and sncRNA2 plasmids used in these studies. (A) Plasmid containing the 1.5-kb LAT (pGEM5317) and its promoter has been described previously (17, 18). Shaded boxes indicate sncRNA1 and sncRNA2 sequences within LAT. sncRNA1 (62 nt) (B) and sncRNA2 (36 nt) (C) were generated and inserted into the pSilencer and pcDNA3.1-FLAG plasmids. LAT sequences lacking ΔsncRNA1 sequence (D), ΔsncRNA2 sequence (E), or both sequences (E) and under the LAT promoter were generated and inserted into the pUC57 plasmid. sncRNA1 TTG (F) and sncRNA2 TTG (G) mutant constructs were established by inserting a 3,007-bp region of LAT corresponding to LAT 118641–121660 and containing a single base pair mutation of ATG to TTG in either sncRNA1 or sncRNA2 and inserted into pUC57. All constructs except those in panels B and C are under the LAT promoter.