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. 2022 Feb 9;96(3):e01985-21. doi: 10.1128/jvi.01985-21

FIG 8.

FIG 8

Computational modeling predicts binding of sncRNA1 and sncRNA2 to mouse HVEM promoter. (A) Mouse HVEM promoter sequence corresponding to nucleotides 539/−478 upstream of the HVEM ATG start codon is predicted to bind to sncRNA1 with a free energy of binding of −50.6 kcal/mol. (B) mHVEM promoter sequence corresponding to −484/−421 upstream of the HVEM start codon is predicted to bind to sncRNA2 with a free energy of binding of −19.9 kcal/mol. Mfe refers to free energy of binding. Red boxes show the position of the ATGs in sncRNA sequences. Schematic diagram of (C) the wild-type mouse HVEM promoter construct, (D) the mouse HVEM promoter construct lacking the predicted sncRNA1 binding site, (E) the mouse HVEM promoter construct lacking the predicted sncRNA2 binding site, and (F) the mouse HVEM promoter construct lacking both the predicted sncRNA1 and sncRNA2 binding sites.