FIG 2.

Tax increases Pim-1 and Pim-3 kinase expression, while inhibiting Pim-2. (A) 293T cells were transfected for 48 h with Tax or HBZ expression vectors and then probed for Pim kinases expression. Actin served as a loading control. (B) Comparison of Tax expression between 293T-Tax expressing cells and Tax-positive, HTLV-1 cell lines, MT4 and C91PL. Actin served as a loading control. (C) Western blot analysis of whole cell lysate of Jurkat and K562 cells for Pim expression. Actin served as a loading control. (D) Jurkat TET-Tax lines were induced with or without doxycycline for 6 days. Representation of at least two independent experiments is shown. Gene expression was normalized to GAPDH expression and performed at least twice. (E) Western blots for Pim kinase expression following induction of Jurkat TET-Tax lines. Cells were induced with or without doxycycline for 6 days. Actin served as a control. (F) Knock-down of Tax expression in ATLT cells. Cells were transduced with a Tax shRNA and 72 h later, cells were analyzed for Tax and Pim kinase expression. Experiments were performed at least twice. Gene expression was normalized to GAPDH.