FIG 3.

Tax transduction of normal PBMCs induces Pim kinases early after infection. (A–B) Four Tax transduced cultures of PBMCs and two cycling PBMCs (cPBMCs) were compared for Pim kinase expression (A) or Tax expression (B). Gene expression was normalized to GAPDH expression and performed at least twice. For Tax expression, Tax transduced cultures were compared with the MT4 cell line. (C–D) Tax transduced PBMCs express Pim kinases in as little as 4- or 7-weeks post-transduction. Two separate, healthy donor PBMCs were transduced with Tax and grown for 4 or 7 weeks. Gene expression of Pim kinases (C) and Tax (D) were examined at both time points and compared with non-transduced PBMCs at 4 weeks. Gene expression was normalized to GAPDH.