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. 2022 Feb 9;10(1):e02066-21. doi: 10.1128/spectrum.02066-21

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Copyright © 2022 Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 10 — (A) EMSA of the interaction between BbBrlA and promoter of Bbcmr1. The promoter region of Bbcmr1 (642 bp to 692 bp upstream of ATG) was used as labeled probe, and unlabeled probe was added in a 200-fold excess. (B) Yeast one-hybrid analysis of BrlA binding the promoter of Bbcmr1. (C) EMSA of the binding activity of BbCmr1 with the promoter of BbbrlA (586 bp to 637 bp upstream of ATG). Each lane contained 10 ng labeled probe, purified protein (0.5 2 μg) or purified protein (2 μg) and unlabeled probe (200 800–fold excess) were added in reactions. (D) Yeast one-hybrid analysis for the interaction between BbCmr1 and the promoter of BbbrlA. (E) A4GA3 was the possible binding site of BbCmr1.