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Bismuth nitrate suppresses the evolution of tigecycline resistance. (A) MPC values of tigecycline against E. coli B3-1 in the presence of different concentrations of bismuth nitrate ranging from 0 to 1,000 μg/mL. (B and C) Serial passages of two tet(X)-positive isolates at subinhibitory concentrations of tigecycline or the combination of tigecycline and bismuth nitrate. The MIC values were measured at every four passages.
