TABLE 1.
Cryo-EM data collection, refinement, and validation statistics
| RVFV L | |
|---|---|
| Data collection and processing | |
| Magnification | 64,000 |
| Voltage (kV) | 300 |
| Electron exposure (e-/Å2) | 50 |
| Defocus range (μm) | -1.0 to −3.0 |
| Pixel size (Å) | 1.08 |
| Symmetry imposed | C1 |
| Final particle images (no.) | 554K |
| Map resolution (Å) | 3.6 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 3.5−4.7 |
| Refinement | |
| Initial model used (PDB code) | 6L42 |
| Map sharpening B factor (Å2) | −180 |
| Model composition | |
| Nonhydrogen atoms | 9,819 |
| Protein residues | 1400 |
| B factors (Å2) | |
| Protein | 103.86 |
| R.m.s deviation | |
| Bond lengths (Å) | 0.006 |
| Bond angles (°) | 1.078 |
| Validation | |
| MolProbity score | 1.28 |
| Clashscore | 5.25 |
| Poor rotamers (%) | 0.00 |
| Ramachandran plot | |
| Favored (%) | 100 |
| Allowed (%) | 0.00 |
| Disallowed (%) | 0.00 |