FIG 1.

Biological characteristics of the H240R protein (pH240R). (A) Multiple sequence alignment of pH240R of 21 ASFV isolates. To assess the nature of the replacements at multiple residues, conservation scores based on the biological properties of each amino acid were included, with lower scores associated with more divergent replacements. Analysis was conducted on Jalview software version 2.11.1.4, using the ClustalW algorithm. (B) H240R gene transcriptional dynamics. Average cycle threshold values of the ASFV HLJ/2018-infected primary porcine alveolar macrophages (MOI of 5) at 1, 4, 7, 10, and 24 hpi were examined by quantitative reverse transcription-PCR analyses using primers against H240R, CP204L, B646L, and GAPDH.