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. 2022 Feb 9;96(3):e01614-21. doi: 10.1128/JVI.01614-21

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Copyright © 2022 Utrilla-Trigo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — Cellular immune responses against BTV in mice immunized with MVA-NS2, MVA-NS2-Nt, and MVA-NS2-Ct. (A) Indirect immunofluorescence of DF-1 cells infected with MVA-NS2, MVA-NS2-Nt, MVA-NS2-Ct, or noninfected (control). NS2, NS2-Nt, and NS2-Ct (red) were detected using MAb 23H6 α-NS2. Nuclei were stained with DAPI. Scale bars 20 μm. (B, C) Interferon gamma ELISPOT assay. Splenocytes of IFNAR(-/-) mice immunized with MVA-NS2, MVA-NS2-Nt, or MVA-NS2-Ct were stimulated with the recombinant proteins NS2-Nt (B) or NS2-Ct (C). Points represent individual values for each mouse, bars represent the mean values of each group and errors bars represent SD. Asterisks denote significant differences between stimulated splenocytes (P < 0.05) (The Mann–Whitney U test).