FIG 6.

Humoral responses against BTV elicited by MVA and ChAdOx1 in IFNAR(-/-) mice. (A, B) Induction of IgG NS1 and NS2 antibodies, respectively, by indirect ELISA in vaccinated animals. Sera dilution 1:50. (C) Neutralizing antibodies titers against BTV in immunized animals by plaque reduction neutralization assay. Cut-off: 0.69 (log 5). Hyperimmune serum: serum from mice infected with BTV-4M. Results were expressed as optical densities (ODs) measured at 450 nm. Bars represent the mean values of each group and error bars represent SD. Asterisks denote significant differences between immunized and control mice (P  <  0.05) (The Mann–Whitney U test). * P value  <  0.05.