Percentages of lymphocytes and neutrophils in blood from immunized sheep after challenge with BTV-4M. Blood of nonimmunized and immunized sheep were analyzed in an autohematology analyzer (BC-5300 Vet; Mindray, China) and the percentage of lymphocytes (A) and neutrophils (B) based on the total white blood cells were analyzed at days 0, 1, 6, 11, and 13 postinfection. Points indicate the mean value of each group and error bars represent SD. * P value < 0.05, ** P value < 0.002, using two-way ANOVA (post hoc Tukey test for multiple comparisons).