TABLE 2.
Primers designed to generate rMVA, rChAdOx1, and rBac
| Primer | Sequence |
|---|---|
| Fw-XmaI-NS2-1a | 5′ CGcccgggATGGAGCAAAAGCAACGTAG 3′ |
| Rs-XmaI-NS2-1062* | 5′ CGcccgggCTAAACGCCGACCGGCAATA 3′ |
| Rs-XmaI-NS2-531* | 5′ CGcccgggCTACGCCACGCTTTGAACTTG 3′ |
| Fw-XmaI-NS2-532* | 5′ CGcccgggATGCCAAGGGAAGAATCACGC 3′ |
| Fw-2A-NS1b | 5′-cttgctgcgcgtatgtctcagatgtggatggaatatGCCCCTGTGAAGCAG-3′ |
| Rs-2A-NS2-Ntb | 5′-gttgcttttgctccatGGGGCCAGGGTTGCTTTCCACGTCGCCGGC-3′ |
| Fw-2A-NS2-Ntb | 5′-GCCGGCGACGTGGAAAGCAACCCTGGCCCCatggagcaaaagcaac-3′ |
| Rs-NS2-Nt-p1990b | 5′-GGGCCCTCTAGATGCATGCTCGAGCGGCCGCctacgccacgctttg-3′ |
| Fw-BamHI-NS2 1c | 5′-CgggatcCGATGGAGCAAAAGCAACGTAG-3′ |
| Rs-NotI-NS2-531c | 5′-CGgcggccgcCTACGCCACGCTTTGAACTTG-3′ |
| Fw-BamHI-NS2-532c | 5′-CgggatcCGATGCCAAGGGAAGAATCACGC-3′ |
| Rs-NotI-NS2-1062c | 5′-CGgcggccgcCTAAACGCCGACCGGCAATA-3′ |
a
Primers designed to generate pSC11-NS2, pSC11-NS2-Nt, and pSC11-NS2-Ct. XmaI restriction site is represented by lowercase letters.
b
Primers designed to generate rChAdOx1. Complementary sequences are shown in capital letters. Lowercase letters represent the overlapping sequences.
c
Primers designed to generate BAC-NS2-Nt and BAC-NS2-Ct. BamHI and NotI restriction sites are represented by lowercase letters.