FIG 4.

Venn diagram displaying the distribution of DEGs in low-virulent 3249-infected piglets at each time point (A). Gene ontology (GO) analysis of 96 overlapped DEGs in response to 3249 strain infection at 6–8–13 dpi. Table lists the top terms of GO biological processes (BPs) and immune system processes (ISPs) enriched with 96 overlapped DEGs (B). Pattern of expression of representative genes (CCL2, CCR5, EDN1, GBP1, GBP7, JAK2) for the most relevant pathways (C). Functional network of BPs and ISPs pathways for the module were visualized in Cytoscape with ClueGo and CluePedia (D). Only the statistically significant terms (FDR < 0.05) in each group are represented. Terms are displayed as nodes (filled circle) linked by edges (lines) based on their kappa value (≥ 0.4), where only the label of the most significant term per group is shown.