TABLE 5.
Primer sequences of Hub genes used to validate RNA-seq analysis
| Gene | Sequences | Reference |
|---|---|---|
| GAPDH | F 5′-ACATGGCCTCCAAGGAGTAAGA-3″ R 5′-GATCGAGTTGGGGCTGTGACT-3″ |
105 |
| CD274 (PD-L1) | F 5′-GTGGAAAAATGTGGCAGCCG-3″ R 5′-TGCTTAGCCCTGACGAACTC-3″ |
Self-designed |
| CTLA4 | F 5′-TCTTCATCCCTGTCTTCTCCAAA-3″ R 5′-GCAGACCCATACTCACACACAAA-3″ |
106 |
| HACVR2 (TIM3) | F 5′-TTCGACGGGAGCAGTAAAGC-3″ R 5′-AGGGCAGGACACAGTCAAAG-3″ |
Self-designed |
| LAG3 | F 5-CTCCTCCTGCTCCTTTTGGTT-3″ R 5′-CAGCTCCCCAGTCTTGCTCT-3″ |
106 |
| PDCD1 (PD-1) | F 5′- AGCCCAAGCACTTCATCCTC-3″ R 5′- TGTGGAAGTCTCGTCCGTTG-3″ |
Self-designed |
| IL10 | F 5′- TGAGAACAGCTGCATCCACTTC -3″ R 5′- TCTGGTCCTTCGTTTGAAAGAAA -3″ |
107 |
| TBX21 (T-bet) | F 5′- TGCAGTCCCTCCATAAGTACCA -3″ R 5′- GCCTCTGGCTCACCATCATT -3″ |
108 |
| CD28 | F 5′-CCCCTCAATTCAAGTAACAGGAAAC-3″ R 5′- ATGCCCGGAACTCCTTTGAG-3″ |
Self-designed |
| TNFRSF9 | F 5′-TTGCCAGCAAGGTCAAGAGT-3″ R 5′- AGCCAAAGAACAGTCCGTCC-3″ |
Self-designed |
| GZMB | F 5′-GCCCCTACATGGCGTATCTTC-3″ R 5′- ACGTTGATTGAGCTTCCCCA-3″ |
Self-designed |
| SELL | F 5′-CCTAGTCCGATATGTCAAAAACTGG-3′ R 5′- TCATCCATGCTTCTCTGAGACTT-3″ |
Self-designed |
| CCR7 | F 5′-TTGTCATTTTCCAGGTGTGCC-3″ R 5′- GGAGTACATGACCGGGAGGA-3″ |
Self-designed |