Fig. 7. Glucose tolerance at multiple ages in β-cell specific Insr knockout mice fed 2 different diets.

a Tail blood glucose levels (mM) from glucose tolerance tests of LFD-fed females (control (black lines, solid circles), βInsr^HET (light pink, open squares), βInsr^KO, (dark red lines, solid triangles)); (n_4week = 16, 16, 14; n_9week = 23, 25, 17; n_21week = 12, 15, 12; n_39week = 8, 12, 10) and males (control (black lines, solid circles), βInsr^HET (light blue, open squares), βInsr^KO (male = dark blue, solid triangles); (n_4week = 10, 18, 5; n_9week = 17, 30, 11; n_21week = 8, 14, 8; n_39week = 9, 7, 13). b Tail blood glucose levels (mM) from glucose tolerance tests of HFD-fed female (control (black lines, solid circles), βInsr^HET (bright pink, open squares), βInsr^KO (purple lines, solid triangles)); (n_9week = 17, 17, 14; n_21week = 12, 14, 12 n_39week = 14, 16, 10; n_54week = 10, 13, 11) and male (control (black lines, solid circles), βInsr^HET (bright green, open squares), βInsr^KO (dark green lines, solid triangles)); (n_9week = 7, 16, 9; n_21week = 7, 16, 8; n_39week = 8, 14, 8; n_54week = 6, 11, 7) mice. All mice received a glucose bolus of 2 g glucose/kg body mass (i.p) except older HFD-fed males, which received only 1 g glucose/kg body mass (i.p). *p-values are italicized when βInsr^KO was compared to controls, p-values are italicized and underlined when βInsr^HET was compared to controls. Data were analysed by a fitted mixed-effects model with correction for multiple comparisons using Dunnett’s method. All animals/samples were handled in a strictly blinded manner. All data are presented as mean values ± SEM. Source data are provided as Source Data file.