FIG 3.

Generation of PLSCR1-KO 36T-3 cells. 36T-3 and CRISPR/Cas9-generated PLSCR1-KO 36T-3 (PLS1KO-A and PLS1KO-B) cells were treated with or without 3,000 units/mL IFN-α-2b for 16 h. Total cell lysates were prepared using RIPA buffer (Sigma-Aldrich), and a total of 4 μg of total cell lysates were subjected to SDS-PAGE. IB was performed using an anti-PLSCR1 antibody to detect endogenous PLSCR1, an anti-ISG15 antibody to detect endogenous ISG15, or an anti-actin antibody to detect endogenous β-actin.