FIG 1.

A. baumannii inhibits growth of Staphylococcus and Corynebacterium species under iron-limited conditions. (A) A. baumannii 17978 was spotted on a lawn of the indicated Staphylococcus or Corynebacterium species, grown on nutrient rich agar alone (control) or with 200 μM the iron chelator 2,2’-dipyridyl (Iron limited). A. baumannii generated a zone of clearance against all strains except C. propinquum ATCC 51488 under iron limited conditions. Experiments were performed in triplicate. A representative image is shown. (B) Quantification of A. n = 3 per competition for inhibited bacteria. The error bars represent the standard error of the mean. (C) Multiple laboratory and clinical A. baumannii isolates inhibit the growth of S. hominis under iron depleted conditions. (D) A. baumannii 17978 inhibits representative Staphylococcus and Corynebacterium species at pH 6.3 (Buffered using 50 mM MES.) in addition to standard culture pH conditions.