FIG 2.

A. baumannii AB5075 Acinetobactin mutants exhibit decreased inhibition of multiple bacteria. (A) Growth of the parent and transposon mutant strains of A. baumannii AB5075 on 300 μM 2,2’-dipyridyl. (B) Growth curves of wild-type and transposon mutant strains of A. baumannii AB5075 grown in 300 μM 2,2’-dipyridyl tryptic soy broth. Growth curves were conducted in triplicate and plotted on a logarithmic scale. The error bars represent the standard error of the mean. (C) A. baumannii AB5075 transposon mutant and wild-type zones of inhibitions on iron limited media. Zone of inhibition assays were conducted in triplicate. (D) Quantification of the zones of inhibition in C. The error bars represent the standard error of the mean. An ANOVA with multiple comparisons, comparing each mutant to the control wild-type strain, was used to assess significance. (E) A. baumannii AB075 wild-type and three basD:T26 isogenic mutant zones of inhibition and quantification. The error bars represent the standard error of the mean. An ANOVA with multiple comparisons was used to assess significance. (⁕⁕, P ≤ 0.01; ⁕⁕⁕, P ≤ 0.001; ⁕⁕⁕⁕, P ≤ 0.0001).