FIG 1.

Phylogenetic relationships between the species of the Bacillus cereus group with Bacillus subtilis subsp. subtilis used as the outgroup. The tree was obtained by the neighbor-joining method based on a comparison of the concatenated sequences of seven housekeeping genes (glpF, gmk, ilvD, pta, pur, pycA, tpi). The Kimura two-parameter distance measure was used as implemented in MEGA X (28). Values above the lines indicate how the tree’s branches are supported by the results of bootstrap analysis (×100 replicates) (only values greater than 70% are shown). The letters “p” and “l” under the lines indicate branches that were also found by the maximum parsimony method and maximum likelihood method, respectively (28). Scale bar, accumulated changes per nucleotide. Asterisks indicate species published but not yet validated.