FIG 3.
Overexpressing HOXA3 downregulated HO-1 expression and promoted PRRSV replication. The lentivirus-mediated overexpression method was used to enhance the expression of HOXA3 in MARC-145 cells. (A to C) MARC-145-HOXA3 cells were infected with PRRSV at an MOI of 0.1. Samples were collected at 24 and 36 hpi. HOXA3 (A), HO-1 (B), and ORF7 (C) mRNA levels were determined by RT-qPCR. (D) The expression of HOXA3-HA, HO-1, PRRSV N, and GAPDH were detected by Western blotting. (E) Cell culture supernatants were collected at the indicated times. TCID50 assay was performed to determine the levels of supernatant virus production. (F) The expression of the N protein was determined by IFA at 36 hpi, with WT MARC-145 cells infected with PRRSV included as a control. Results are expressed as means ± SD of three independent replicates. Statistically significant values were denoted as follows: *, P < 0.05; ***, P < 0.001.