FIG 6.

Deficiency of HOXA3 promoted nuclear translocation of IRF3. (A and B) HOXA3^−/−-MARC-145 cells were infected with PRRSV at an MOI of 0.1. Cells were collected at 36 and 48 hpi after transfection of poly(I·C) for 12 h. Cytoplasmic and nuclear extracts were prepared and subjected to Western blotting. The expressions of p-IRF3, IRF3, HO-1, GAPDH, and histone H3 were detected. (C) MARC-145 cells transfected with siNC and siHOXA3 no. 1 for 24 h were infected with PRRSV at an MOI of 0.1 for 48 h. The subcellular localization of IRF3 was determined by IRF3 intracellular staining, and confocal fluorescence images were captured.