Fig. 2. The dynamic changes of single-cell metabolic profiles after interaction with NK cells. (a) The heat map of two groups of single HepG2 cells with different ratios of E : T. In one group, 162, 102, 201, 195, and 200 cells were detected with the ratio of E : T from 0 : 1, 1 : 1, 3 : 1, and 5 : 1 to 10 : 1. In the other group 186, 160, 143, 115, and 179 cells were detected. (b) The normalized MS profiles of HepG2 cells with the ratio of E : T from 0 : 1 to 10 : 1. (c) The metabolic differences with and without co-culture with NK cells. (d) The consecutive distribution of HepG2 cells with different ratios of E : T from 0 : 1 to 10 : 1, from the single-cell metabolic profile analysis.