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. 2022 Feb 8;13(2):121. doi: 10.1038/s41419-022-04588-0

Fig. 1. Knockdown of MYOCD triggered autophagy in HA-VSMCs.

Fig. 1

A The expression of MYOCD was detected in HA-VSMCs transfected with shRNA specifically targeting MYOCD by western blotting (*P < 0.05, **P < 0.01, n = 3). B The expression of contractile proteins ACTA2 and SM22 was detected in MYOCD-knockdown HA-VSMCs by western blotting (**P < 0.01, n = 3). C The expression of OPN and PCNA was tested in MYOCD-knockdown HA-VSMCs by western blotting (*P < 0.05, n = 3). D Cellular proliferation was evaluated in MYOCD-knockdown HA-VSMCs by EdU staining (**P < 0.01, n = 3). E The expression of beclin1, P62, and LC3 was tested in MYOCD-knockdown HA-VSMCs by western blotting (*P < 0.05, n = 3). F Bafilomycin A1 (0.1 μg/mL) was added to control or MYOCD-knockdown HA-VSMCs 1 h prior to harvest, and accumulation of P62 and LC3 was measured by western blotting (*P < 0.05,**P < 0.01, n = 3). G Representative images and quantification of GFP-LC3 puncta in MYOCD-knockdown HA-VSMCs transfected with GFP-LC3 vector in the presence or absence of bafilomycin A1 (*P < 0.05,**P < 0.01, n = 3).